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Rapid detection of HPV16/18 based on a CRISPR-Cas13a/Cas12a dual-channel system

Anal. Methods, 2022, Advance Article
DOI: 10.1039/D2AY01536F, Paper
Xue Zheng, Yuankun Li, Mingzhu Yuan, Yue Shen, Shuaiyin Chen, Guangcai Duan
Orthogonal cleavage of DNA and RNA reporters by target-activated Cas12a/Cas13a induces two-colored fluorescence emission (JOE-FAM), which can be detected using a standard fluorometer or observed by a dual-channel visualization detection platform.
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Water-soluble long afterglow carbon dots/silica composites for dual-channel detection of alkaline phosphatase and multi-level information anti-counterfeiting

Anal. Methods, 2022, Advance Article
DOI: 10.1039/D2AY01587K, Paper
Xiangying Sun, Wei He, Bin Liu
The CDs/SiO2 composites were used in detection of the dual-optical mode alkaline phosphatase and multi-level information anti-counterfeiting.
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Molecularly Imprinted Polymers as effective capturing receptors in a pseudo-ELISA Immunoassay for Procalcitonin detection in Veterinary Species

Anal. Methods, 2022, Accepted Manuscript
DOI: 10.1039/D2AY01175A, Paper
Open Access Open Access
Creative Commons Licence&nbsp This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
FEDERICA BATTAGLIA, Francesca Bonelli, Micaela Sgorbini, Luigi Intorre, Maria Minunni, Simona Scarano, Valentina Meucci
In this study, a new sandwich-type immunoenzymatic assay, based on a molecularly imprinted polymer (MIP) as an artificial antibody (pseudo-ELISA), was developed for the determination of procalcitonin (PCT) in veterinary...
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Electrochemical biosensor for the detection of aflatoxin B1 based on specific aptamer and HCR biological magnification

Anal. Methods, 2022, Accepted Manuscript
DOI: 10.1039/D2AY01682F, Paper
Hongyan Zhang, Siying Ye, Lishan Huang, Shen Fan, Weiwei Mao, Yijin Hu, Yuyan Yu, Fengfu Fu
Aflatoxin B1 (AFB1) is a highly toxic mycotoxin, which causes severe acute or cumulative poisoning. Therefore, it is important to fabricate sensitive and selective detection method of AFB1 for the...
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An allosteric DNA switch-mediated catalytic DNA circuit for ratiometric and sensitive nucleic acid detection

Anal. Methods, 2022, Accepted Manuscript
DOI: 10.1039/D2AY01751B, Paper
Xiaofan Zhang, Shunjun Xie, Xue Chen, Li Wang, Fang Li, Shufeng Liu
Herein, a new allosteric DNA switch-mediated catalytic DNA circuit reaction strategy was proposed for ratiometric and sensitive nucleic acid detection. The sensing system was based on two DNA hybrid probes...
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Rapid and comprehensive identification of chemical constituents in Mai-Luo-Shu-Tong pill by UHPLC-Q-Orbitrap HRMS combined with a data mining strategy

Anal. Methods, 2022, Advance Article
DOI: 10.1039/D2AY01453J, Paper
Xiangyu Zhang, Yaojuan Chu, Mengli Wang, Yingying Shi, Lihua Zuo, Zhuolun Li, Jiyun Liu, Jian Kang, Shuzhang Du, Bing Li, Zhi Sun, Xiaojian Zhang
Mai-Luo-Shu-Tong pill is an effective traditional Chinese medicine formula for the treatment of superficial thrombophlebitis, but it was insufficiently chemically scrutinized.
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Different approaches for estimation of expanded uncertainty of an analytical method developed for determining pharmaceutical active compounds in wastewater using solid-phase extraction and liquid chromatography coupled with tandem mass spectrometry method

Anal. Methods, 2022, Accepted Manuscript
DOI: 10.1039/D2AY01676A, Paper
João Rodrigues, Sofia Silva, Vitor Cardoso, Maria João Benoliel, Cristina Almeida
Although the evaluation of the uncertainty of an analytical method is a mandatory step in the method's validation, its applicability to the monitoring of trace compounds in complex samples is...
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Development of multi-residue gas chromatography coupled with mass spectrometry methodologies for the measurement of 15 chemically different disinfection by-products (DBPs) of emerging concern in drinking water from two different Portuguese water treatment plants

Anal. Methods, 2022, Advance Article
DOI: 10.1039/D2AY01401G, Paper
Raquel S. Chaves, Joao E. Rodrigues, Miguel M. Santos, Maria J. Benoliel, Vitor V. Cardoso
MA-based methods were validated to measure 15 chemically different DBPs in drinking water matrices. The measurement of target DBPs in drinking waters suggested occurrence levels lower than the obtained MQLs.
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Naphthalimide-tyrosine-based dicationic amphiphile for intracellular ‘turn-on’ simultaneous detection of ATP and CTP

Anal. Methods, 2022, Accepted Manuscript
DOI: 10.1039/D2AY01550A, Paper
Poonam Sharma, Sugandha Kumar, Amandeep Walia, Satwinder Singh Marok, Vanita vanita, Prabhpreet Singh
We have developed a new naphthalimide-based amphiphile (YN-1) for the simultaneous detection of ATP and CTP. In YN-1, the cationic tyrosine-linked polyamine (+2 charge, hydrophilic unit) is appended at -peri...
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Highly sensitive competitive aptasensor for AFB1 detection based on exonuclease-assisted target recycling amplification strategy

Anal. Methods, 2022, Accepted Manuscript
DOI: 10.1039/D2AY01617F, Paper
Hongyan Zhan, Si Yang, Chenxi Li, Rong Liu, Wenliang Chen, Xiaoli Wang, Yansong Zhao, Kexin Xu
Aflatoxin B1 (AFB1) is a typical mycotoxin found in agricultural products, which poses a huge danger for both humans and animals. The accurate and rapid measurement of AFB1 is essential...
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