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Poly-thymine-mediated fluorescence recovery of N-CQDs for turn-on sensing of APE1 activity in breast cancer cell lysates

Anal. Methods, 2026, 18,3612-3623
DOI: 10.1039/D6AY00468G, Paper

Xinjuan Xiong, Yanning Li, Minghui Zhao, Juan Zhao, Shulong Xiong, Zhao Sun
A label-free turn-on fluorescent biosensor is developed for APE1 activity detection based on APE1-gated release of poly-T strands from a constrained DNA duplex, which competitively displaces Ag⁺ from N-CQDs, recovering fluorescence.
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Post-synthetic and in situ engineering of metal nanoclusters for enhanced stability and multifunctional applications

Anal. Methods, 2026, 18,3445-3464
DOI: 10.1039/D6AY00154H, Critical Review

Ritika Mittal, Nancy Gupta
Engineering metal nanoclusters via post-synthetic and in situ strategies enhances stability and photoluminescence while enabling new functionalities. This review critically evaluates these approaches, associated properties, and applications.
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A highly sensitive SERS sensor for malachite green detection based on spiky Fe3O4@Au supraparticles

Anal. Methods, 2026, 18,3591-3599
DOI: 10.1039/D6AY00166A, Paper

Hang Ye, Yanke Wang, Hufu Chen, Tao Huang, Shengnan Zhan
A novel label-free analytical strategy based on surface-enhanced Raman spectroscopy (SERS) has been developed for the detection of malachite green (MG) using spiky Fe₃O₄@Au supraparticles (Fe₃O₄@AuSPs).
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A ligation DNAzyme-driven catalytic hairpin assembly strategy for miRNA detection

Anal. Methods, 2026, 18,3634-3644
DOI: 10.1039/D6AY00460A, Paper

Hongbo Zhang, Yugang Xiao, Zhuoer Zeng, Di Zhang, Zhirui Song, Xiaoke Wu, Qubo Zhu
A ligation DNAzyme-driven catalytic hairpin assembly (LiD-CHA) strategy enables low-background, highly sensitive miRNA-21 detection by regenerating new trigger strands. The method shows good specificity and reliable performance in serum samples.
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Analytical characterization of bulk R-Salt and its odor profile to establish a framework for canine detection

Anal. Methods, 2026, 18,3490-3501
DOI: 10.1039/D6AY00077K, Paper

Janet Crespo-Cajigas, Melissa J. Clinard, Shawanda Roberts, Dawn M. Mills
TD-GC-MS was used to characterize the headspace of an improvised explosive, R-Salt. This study provides foundational data and methodology that is essential for the development and validation of canine training aids and field vapor sampling devices.
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Highly sensitive identification of illegal cooking oil by surface-enhanced Raman spectroscopy combined with acyl nucleophilic substitution reaction to detect capsaicin

Anal. Methods, 2026, 18,3570-3580
DOI: 10.1039/D6AY00465B, Paper

Shengping Han, Guican Deng, Yonghua Bao, Yanping Hong, Jiaxin Hong, Luying Duan, Huaying Du, Zhimei Tan, Chunrong Wang
A simple method for capsaicin detection based on acyl nucleophilic substitution coupled with surface-enhanced Raman spectroscopy has been developed for the rapid screening and identification of gutter oil adulteration.
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Unraveling the alteration of chemical compositions in Cordyceps and its host larvae using mass spectrometry imaging

Anal. Methods, 2026, 18,3581-3590
DOI: 10.1039/D6AY00457A, Paper

Shipeng Sun, Yuchen Zou, Weiwei Tang, Zhengming Qian, Bin Li
This work investigates the spatial alterations of chemical compositions across the host larva, the stroma and sclerotium of Cordyceps using MALDI MSI and LC-MS.
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Species identification of animal glue adhesives on polychrome cultural heritage: an integrated analytical approach combining gel sampling, ATR-FTIR spectroscopy, and machine learning

Anal. Methods, 2026, 18,3645-3656
DOI: 10.1039/D6AY00017G, Technical Note

Xinyu Xie, Wanru Wang, Qin Huang, Wenyuan Zhang, Xinyue Zhu, Xiaoyan Liu, Haixia Zhang
Schematic of the integrated workflow for species identification of animal glue adhesives, including hydrogel sampling, ATR-FTIR analysis, and supervised machine learning classification.
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Enzyme-free and product-accelerated DNA walker cascade for ultrasensitive detection of tic disorder-associated microRNA

Anal. Methods, 2026, 18,3515-3522
DOI: 10.1039/D6AY00424E, Paper

Wei Li, Xiaobin Dou
Tic disorders (TDs) are prevalent neuropsychiatric conditions in children, yet early diagnosis remains challenging due to limited availability and high sequence homology of associated microRNA biomarkers.
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Chemometric evaluation of the CUPRAC assay for propolis quality control: a decision-making framework for deciphering antioxidant paradoxes

Anal. Methods, 2026, 18,3557-3569
DOI: 10.1039/D6AY00251J, Paper

Open Access

&nbsp This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Mehmet Beykaya, Aslı Elif Tanuğur Samancı, Taylan Samancı, Elif Önder Yorulmaz
The antioxidant capacity of propolis is traditionally inferred from single compositional indicators such as total phenolic (TPC) or flavonoid content (TFC).
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