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Electrochemical aptamer-based sensor for cerebrospinal fluid detection

Anal. Methods, 2025, 17,9649-9658
DOI: 10.1039/D5AY01335F, Paper

Xinlei Chen, Mehmet Senel, Junye Li, Arash Abiri, Andrej Lupták, Frank P. K. Hsu, Edward C. Kuan, Michelle Khine
We developed a new class of E-AB biosensors capable of detecting target analytes even in the absence of complete biomarker identification and outlined essential optimization strategies to enhance signal suppression performance.
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A capillary SERS sensor based on CRISPR/Cas13a and DS Au-AgNRs for detecting miRNA-221 in serum of hepatocellular carcinoma patients

Anal. Methods, 2025, 17,9627-9637
DOI: 10.1039/D5AY01517K, Paper

Zixun Wang, Dong Zhang, Yuxuan Wu, Xu Ye, Yayun Qian, Fengsong Chen
In the presence of the target, ssDNA modified with Cy5 was cleaved and moved away from the substrate, with a significant decrease in Raman signal compared to that in the absence of the target.
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Development of a new procedure for selecting internal standards for gas chromatography/mass spectrometry-based plasma metabolome analysis

Anal. Methods, 2025, 17,9683-9688
DOI: 10.1039/D5AY01659B, Technical Note

Shin Nishiumi, Tomonori Yokoyama, Noriyuki Ojima
Mass spectrometry, including GC/MS, LC/MS, and CE/MS, can analyze many different molecules in a single measurement, facilitating metabolome analysis.
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An integrated LC-MS approach to the metabolome profile in the whole-body tissue of adult zebrafish

Anal. Methods, 2025, 17,9618-9626
DOI: 10.1039/D5AY01240F, Paper

Mengmeng Sun, Qiyu Yao, Linjiang Wei, Zhi Zhou
Zebrafish (Danio rerio) has served as a crucial experimental model in biomedical and environmental research. An untargeted metabolomics approach combining HILIC and RPLC was established suitable for the whole-body tissue of adult zebrafish.
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Rapid on-site monitoring of heavy metals in gold-mine tailings via filtered thin-layer deposition and low-power PEDXRF

Anal. Methods, 2025, 17,9608-9617
DOI: 10.1039/D5AY01804H, Paper

Yande Chao, Hao Xue, Jie Chen, Yongjin Li, Zaijie Shi, Jiwei Zhang, Qing Shan, Wenbao Jia
This study enables direct heavy metal quantification in gold mine tailings slurry by integrating optimized filtration (−70 kPa, 60 s) to reduce moisture to ∼20% with a low-power PEDXRF system for rapid on-site analysis.
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Preparation of Fe3O4@Ag for sensitive detection of organic arsonic acids via surface-enhanced Raman spectroscopy

Anal. Methods, 2025, 17,9545-9556
DOI: 10.1039/D5AY01522G, Paper

Xamsiya Nurmamat, Ziqi Zhang, Zhixi Zhao, Yongbin Li, Huaqing Ling, Yufang Ye, Qing Wang
Surface enhanced Raman spectroscopy (SERS) can detect organic arsonic acid contaminants in the environment, but its detection mechanism is less studied.
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Review on novel methods of tear collection for protein analysis

Anal. Methods, 2025, 17,9516-9529
DOI: 10.1039/D5AY00218D, Tutorial Review

Kai Yan Wong, Shuowen Zhou, Marco King In Ma, Chin Long Choi, Zhiting Peng, Yau Kei Chan
This review explores and evaluates novel tear collection methods designed to overcome the limitations of traditional techniques, including issues related to patient discomfort, contamination risks, and procedural inefficiencies.
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Composition and transformation of sulfur-, nitrogen-, and oxygen-containing compounds in the hydrotreating process of a FCC slurry decant oil

Anal. Methods, 2025, 17,9667-9676
DOI: 10.1039/D5AY01687H, Paper

Haidong Li, Xiu Chen, Yahe Zhang, Quan Shi
A FCC slurry decant oil was subjected to catalytic hydrotreating to investigate the molecular transformation of sulfur-, nitrogen-, and oxygen-containing compounds.
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Characterization of volatile compounds in Paeonia lactiflora Pall seed oil by Heracles NEO ultrafast E-nose and GC-IMS under different processing methods

Anal. Methods, 2025, 17,9586-9598
DOI: 10.1039/D5AY01496D, Paper

Jiaxin Liu, Wanting Wang, Zheng Wang, Qingming Zhang, Yuting Zhang, Chengyan Jing, Wencong Huang, Hui Xie, Li Ma, Hongli Yu, Jianqiu Chen, Yonghai Liu, Xingde Zhang
This study characterized the volatile profiles of Paeonia lactiflora seed oil obtained by various extraction and refining methods, and identified key odor markers distinguishing these processing conditions.
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Application of Zernike moments for the quantitative analysis of polycyclic aromatic hydrocarbons based on fluorescence three-dimensional spectra

Anal. Methods, 2025, 17,9638-9648
DOI: 10.1039/D5AY01360G, Paper

Yao-yao Cui, Shao-zhe Wu, Can Cui, Han-bing Wu, Jin-yi Li, Tian Wang
A method combining 3D fluorescence, Zernike moments, and GRNN is proposed for quantifying PAHs like acenaphthene and naphthalene in mixtures.
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