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Rational design of a 1,8-naphthalimide-based fluorescent probe for water detection in organic solvents

Anal. Methods, 2025, Advance Article
DOI: 10.1039/D5AY01604E, Paper

Yanpeng Dai, Haoting Gao, Han Huang
Generally, trace amounts of water in organic solvents are deadly to chemical production and laboratory chemical reactions.
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An improved LC-MS/MS-based assay for the quantification of vancomycin in human plasma and bone tissue from osteomyelitis patients

Anal. Methods, 2025, Advance Article
DOI: 10.1039/D5AY01529D, Paper

Qixian Ling, Yuanyuan Zhang, Zhe Wang, Haotian Ma, Sihan Wang, Yanan Wang, Xin Xiong, Libo Zhao
A rapid, sensitive assay for vancomycin in dual matrices: plasma and bone.
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Untargeted metabolomics pilot study using UHPLC-Q-Orbitrap to investigate potential metabolic markers of Brucella abortus biovars

Anal. Methods, 2025, Advance Article
DOI: 10.1039/D5AY00998G, Paper

Joane M. M. Corrêa, Mauro L. G. de Oliveira, Patrícia G. de Souza, Paulo M. S. Filho, Diego G. Rocha, Adriana N. de Macedo, Adriana F. Faria
This was a pioneering pilot study that investigated metabolic differences in six biovars of Brucella abortus. Two metabolites were putatively identified and may be potential markers for biovar differentiation.
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A nanozyme-based colorimetric platform for total antioxidant capacity evaluation: optimizing vine tea brewing with Fe3O4@Ag@Pt

Anal. Methods, 2025, Advance Article
DOI: 10.1039/D5AY01574J, Paper

Tian Li, Jiaoyuan Fang, Yun Wang, Jiahui Qu, Xiaoling Zhang, Xiangjun Qiu, Liping Zhang
Fe₃O₄@Ag@Pt nanozymes enable rapid, sensitive quantification of vine tea's antioxidant capacity, promoting the application of nanozyme-based sensors in food and medicinal analysis.
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Optimized extraction and analytical protocols for guanitoxin: an organophosphate cyanobacterial neurotoxin of environmental concern

Anal. Methods, 2025, 17,9486-9493
DOI: 10.1039/D5AY01055A, Paper

Kelly A. Fernandes, Fernanda Rios Jacinavicius, Maria Giseli Tomaz Silva Souza, Rafaella Bizo Menezes, Ernani Pinto
This study reports the optimization of cultivation, extraction, and semipurification processes of the guanitoxin-producing ITEP-24 strain, with the goal of developing an analytical standard and supporting the monitoring of related ecological risks.
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Ni/Ag bimetallic nanoparticle-engineered Ti3C2Tx MXene composite as a SERS substrate for trace detection of thiram pesticide

Anal. Methods, 2025, 17,9467-9474
DOI: 10.1039/D5AY01695A, Paper

Jinping Tang, Yuhai Wang, Chunxu Wang, Ziwei Yu, Jin Wang
Surface-enhanced Raman scattering (SERS) has emerged as a powerful analytical tool for trace molecular detection, enabling identification of target analytes through their characteristic vibrational fingerprints.
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Developing and validating a sensitive and fast UPLC-MS/MS method for estimating the in vitro metabolic stability of crenolanib in HLMs: identification of structural alarms related to the in silico toxicity and metabolic lability

Anal. Methods, 2025, 17,9442-9453
DOI: 10.1039/D5AY01691F, Paper

Mohamed W. Attwa, Awadh M. Ali, Haitham AlRabiah, Adnan A. Kadi
Metabolic stability research showed crenolanib's moderate extraction ratio. In silico software suggested that small piperidin-4-amine moiety alterations (99%) during drug development may improve metabolic stability.
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Detection of biomolecular interactions using polyfluorine tracers in diffusion ordered spectroscopy

Anal. Methods, 2025, 17,9422-9431
DOI: 10.1039/D5AY01657F, Paper

Tasneem S. Habib, Abdulrahman M. Alazemi, Laila A. Alhaddad, Abdul-Hamid M. Emwas, Mohamed K. Emira, Fouzi Mouffouk
The interaction of avidin (yellow) with biotin (blue). The tracer molecule pentadecafluorooctanoyl is shown in green spheres.
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Highly efficient Nile red staining for the rapid quantification of microplastic number concentrations using flow cytometry

Anal. Methods, 2025, 17,9381-9393
DOI: 10.1039/D5AY01093D, Paper

Open Access

&nbsp This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Yuki Kuruma, Hiromu Sakurai, Tomoaki Okuda
Swelling-mediated Nile red staining and flow cytometry.
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MALDI mass spectrometry analysis of N-glycans through DMTMM-mediated benzylamidation and Girard's reagent P labeling

Anal. Methods, 2025, 17,9500-9504
DOI: 10.1039/D5AY01494H, Technical Note

Minju Kim, Eun Ju Lee, Sohee Yoon, Hae-Min Park
MALDI-MS-based N-glycan analysis with both DMTMM-mediated benzylamidation and GP labeling allows the sensitive measurement of intact N-glycans released from glycoproteins.
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