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A portable fluorescent lateral flow immunoassay system for ultrasensitive point-of-care detection of multiple illicit drugs

Anal. Methods, 2025, 17,9454-9466
DOI: 10.1039/D5AY01482D, Paper

Weijia Li, Xiaodan Cheng, Dongfeng Wang, Zhijie Tu, Jianwei Xie, Shengqi Wang, Zhen Rong
A portable fluorescent lateral flow immunoassay system was developed for ultrasensitive point-of-care detection of multiple illicit drugs.
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Spectroscopic analytical approaches for kidney disease biomarker detection: advancements and challenges

Anal. Methods, 2025, 17,9286-9303
DOI: 10.1039/D5AY01279A, Critical Review

Wei Wang, Jing Mao, Jian Wang, Xin Yuan, Ke Huang
Schematic illustration of diverse spectroscopic approaches for detecting AKI and CKD biomarkers, including colorimetric, fluorescence, SERS, and CL methods.
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Complementary liquid and gas chromatographic-mass spectrometric methods for detecting residual impurities in Cyrene, a sustainable bio-derived solvent

Anal. Methods, 2025, 17,9345-9356
DOI: 10.1039/D5AY01597A, Paper

Kaushalya Kankani Durage, Ibraam E. Mikhail, Leo Lebanov, Estrella Sanz Rodriguez, Desmond E. Richardson, Andrew A. Gooley, Brett Paull
Liquid and gas chromatographic-mass spectrometric methods for detecting residual impurities in Cyrene.
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Non-invasive biochemical sensing with AI-driven analytics: a comprehensive review of technologies, applications, and future directions

Anal. Methods, 2025, Advance Article
DOI: 10.1039/D5AY01580D, Tutorial Review

Umapathi Krishnamoorthy
Artificial Intelligence (AI) combined with non-invasive biochemical sensing is transforming healthcare monitoring and diagnostics.
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Dual-mode electrochemical–colorimetric immunosensor based on an integrated FeOOH–Au–MB probe for sensitive procalcitonin detection

Anal. Methods, 2025, 17,9338-9344
DOI: 10.1039/D5AY01192B, Paper

Yueyuan Li, Xiaoying Wu, Yunxia Wang, Zhanquan Zhang, Yongfa Zhang, Xinli Lu
A novel dual-mode immunosensor utilizing FeOOH–Au–MB probes enables self-calibration, thereby enhancing the reliability and sensitivity of sepsis biomarker PCT detection, offering a promising tool for clinical diagnosis.
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Development of a surface plasmon resonance biosensor for the quantification of bevacizumab in human serum

Anal. Methods, 2025, 17,9432-9441
DOI: 10.1039/D5AY01767J, Paper

Ningqi Xia, Dan Li, Hao Chen, Xiaofei Wang, Zhenyu Zhou, Sheng Lou, Bin Lu, Diya Lv, Yan Cao
Bevacizumab, a monoclonal antibody targeting vascular endothelial growth factor A (VEGFA), is a widely used anti-angiogenic drug for malignancies.
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Ultrasensitive electrochemical detection of parvovirus B19 DNA by combining CRISPR-Cas12a and multivalent framework nucleic acids

Anal. Methods, 2025, 17,9357-9364
DOI: 10.1039/D5AY01285F, Paper

Jicong Hao, Xueyan Gong, Xueyuan Duan, Weiwei Qin, Haiying Ren, Xuping ShenTu, Zihong Ye, Xiaoping Yu
An ultrasensitive electrochemical biosensor for parvovirus B19V DNA is presented, integrating CRISPR-Cas12a with framework nucleic acids to achieve an amplification-free detection limit of 2.19 fM.
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Automated dual-cartridge solid-phase extraction method for multi-residue determination of veterinary drugs in bovine muscle, liver, fat, and milk

Anal. Methods, 2025, 17,9319-9327
DOI: 10.1039/D5AY01515D, Paper

Ryu Mochizuki, Shizuka Saito-Shida, Maki Saito, Takaaki Taguchi, Tomoaki Tsutsumi
Automated dual-cartridge solid-phase extraction (SPE) using two serial C18 columns with intermediate water addition enabled efficient cleanup and accurate multi-residue determination of veterinary drugs in bovine tissues and milk by LC-MS/MS.
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Functional component analysis of Nekemias grossedentata insect tea using widely targeted metabolomics and the TCMSP database

Anal. Methods, 2025, 17,9328-9337
DOI: 10.1039/D5AY00850F, Paper

Zhengwen Yu, Yuyu Yuan, Lian Xie, Feng Yang, Tingting Zhang, Danmei Liu, Jie Chen
Widely targeted metabolomics combined with TCMSP database analysis revealed metabolic changes during NGIT processing and predicted the biological activities and pharmacokinetic properties of its major components.
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A simple NIR fluorescent probe for detecting pH in actual water samples and cells

Anal. Methods, 2025, 17,9416-9421
DOI: 10.1039/D5AY01593F, Paper

Qing Mei, Rui-Zhen Zhang, Feng Li
The NIR probe exhibited excellent water solubility and enabled the detection of pH in aqueous systems without organic solvents. Furthermore, the probe was employed to monitor the pH in actual water samples and acidity detection within cells.
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